one aspect of determining the desired audience for your website is determining who you want to reach, which requires and understanding of what you have to offer visitors to your site, both now and in the future.
You may have a deep library of "how to" content, great videos, a unique photo gallery, or an awesome tool that people are interested in using. Each of these can be valuable in building a world-class website that does well in the search engines.
The content you have available to you will affect your keyword research and site architecture, as your site content is the major source of information that search engines use to determine what your site about.